Journal: Biomaterials Research

Article Title: Peripheral nerve defects repaired with autogenous vein grafts filled with platelet-rich plasma and active nerve microtissues and evaluated by novel multimodal ultrasound techniques

doi: 10.1186/s40824-022-00264-8

Figure Lengend Snippet: In vivo study at different time point

Article Snippet: Rabbit anti-S100 antibody (1:200, bs-2015R, Bioss) and mouse anti-Neurofilament 200 antibody (1:200, N5389, Sigma) were, respectively, applied as the primary antibodies and incubated in a humidified chamber overnight at 4 °C.

Techniques: In Vivo, Staining, Immunofluorescence, Microscopy

Journal: Biomaterials Research

Article Title: Peripheral nerve defects repaired with autogenous vein grafts filled with platelet-rich plasma and active nerve microtissues and evaluated by novel multimodal ultrasound techniques

doi: 10.1186/s40824-022-00264-8

Figure Lengend Snippet: Histological evaluation of regenerated nerves at 4 weeks. A H&E staining and immunofluorescence staining were performed at middle and distal segment separately. Anti-Neurofilament 200 was used to stain regenerative axons (red) and anti-S100 antibody was used to stain regenerative myelin sheath (green). Nuclei were stained with DAPI (blue). Statistical analyses were performed by calculating B - C the mean density of regenerative axons between groups. * P < 0.05, ** P < 0.01, and n.s. not significant. Micro-T microtissues, PRP platelet-rich plasma

Article Snippet: Rabbit anti-S100 antibody (1:200, bs-2015R, Bioss) and mouse anti-Neurofilament 200 antibody (1:200, N5389, Sigma) were, respectively, applied as the primary antibodies and incubated in a humidified chamber overnight at 4 °C.

Techniques: Staining, Immunofluorescence